Conventional proteomics research relies on three dimensions post proteolytic digestion: retention time, mass-to-charge ratio (m/z), and peptide ion intensity. With the development of Trapped Ion Mobility Spectrometry (TIMS) and Parallel Accumulation Serial Fragmentation (PASEF) techniques, ion mobility dimension was added as the forth separation dimension, making 4D proteomics technology the latest quantitative approach.
Based on Bruker timsTOF Pro2 platform, 4D DIA Proteomics significantly improves the scanning speed and detection sensitivity, as well as enhances 4D astral proteomics analysis performance in terms of identification depth and quantitative accuracy with less sample input.
The Optimal Choice for 4D Proteomics Analysis of Large-Cohort Samples
4D Proteomics
High Sensitivity: Nearly 100% ion utilization, greatly improving detection sensitivity.
Deeper Protein Identification: Protein identification level has increase by 20%, 6000+ cells per needle (200 ng 293T cells).
Higher integrity and reproducibility of protein area.
Smaller Sample Size Required: Suitable for rare and minimal samples: FFPE, tissue biopsies, immune cells……
4D-DIA Proteomics Project Workflow.
4D-DIA Proteomics Sample Requirements
|
Sample type |
Sample |
Recommended Volume |
|
Body fluid |
Plasma. serum |
0.05~0.2 mL |
|
Urine |
50~100 mL |
|
|
Cerebrospinal fluid, synovial fluid, ascites…… |
0.05~5 mL |
|
|
Animal or human milk |
2~50 mL |
|
|
Animal tissue |
Brain, heart, liver, spleen, lungs, kidneys, muscles, skin, or other tissues |
15 mg~2 g |
|
Plant tissue |
Soft tissues of woody and herbaceous plants |
50 mg~2 g |
|
Cells |
Cells |
0.5×107~1×107 |
|
Special sample |
FFPE |
5-10 µm thick, 50 mm2 size, ≥10 slices |
|
Biopsy or puncture tissue |
1-3 needles, visible to the naked eye, the size of millet grains |
* Please ensure proper pre-processing and storage methods for the samples.
* Please contact [email protected] if you have any questions regarding the samples.
4D-DIA Proteomics Analysis Content Display
As a professional proteomics service company, INOMIXO can provide a variety of bioinformatics and custom mRNA service. For more proteomics analysis needs, please contact [email protected].
INOMIXO is an innovative company dedicated to providing multi-omics solutions in the field of life sciences. By integrating AI big data with advanced mass spectrometry multi-omics technology, along with our team of experienced R&D expertise, INOMIXO has developed two primary business segments, academic omics services and biopharmaceutical CRO services, creating a business landscape that provides solid, professional, and cutting-edge omic studies solutions for developing life sciences.
You must be logged in to post a comment.